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proJ [2019-06-28 10:35:34]
function
osmoadaptive de novo production of proline
product
glutamate 5-kinase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]Gene
Coordinates
2,015,733 2,016,848
The protein
Catalyzed reaction/ biological activity
ATP L-glutamate = ADP L-glutamate 5-phosphate (according to Swiss-Prot)Protein family
glutamate 5-kinase family (with [protein|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|ProJ], according to UniProt)Paralogous protein(s)
[protein|5592C5163CF024289B850916516E2C8C7AD43136|ProB] Structure
[PDB|4Q1T] (from ''Burkholderia thailandensis'', 39% identity)Expression and Regulation
Operons
genes
[gene|542871C77BE841AA59A65FFE8A44AB85BF9180F0|proH]-[gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]
description
[Pubmed|21296969]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21784929], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulation
expressed under conditions of osmotic stress [Pubmed|21784929]view in new tabBiological materials
Mutant
GP817 (spc) available in [SW|Jörg Stülke]'s lab1A635 ( ''proJ''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A635&Search=1A635 BGSC]BKE18470 ([gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG, downstream forward: _UP4_TAATTTGCAGAAAAAAACCCBKK18470 ([gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG, downstream forward: _UP4_TAATTTGCAGAAAAAAACCCReferences
Reviews
Original publications
21296969,25344233,21784929,27766092,28752945